| Microorganism name |
Saccharomyces cerevisiae
|
| Target proteins |
Ovalbumin (Jin et al., 2024)
|
| Wild-type or GMO |
GMO (Jin et al., 2024)
|
| Production mode (intracellular/extracellular) |
Extracellular (Jin et al., 2024)
|
| Protein yield (g/L or g/g?) |
0.132 g/L (Jin et al., 2024)
|
| Temperature used in study |
30°C (Jin et al., 2024)
|
| pH used in study |
pH 5.5 (Jin et al., 2024)
|
| C & N source |
Glucose, yeast extract (Jin et al., 2024)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies |
NA |
| Publications/references |
-
Jin, K. C., Seo, S., & Kim, S. (2024). Animal-free production of hen egg ovalbumin in engineered Saccharomyces cerevisiae via precision fermentation. International Journal of Biological Macromolecules, 271, 132479. https://doi.org/10.1016/j.ijbiomac.2024.132479
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Gomes, A. M. V., Carmo, T. S., Carvalho, L. S., Bahia, F. M., & Parachin, N. S. (2018). Comparison of Yeasts as Hosts for Recombinant Protein Production. Microorganisms, 6(2), 38. https://doi.org/10.3390/microorganisms6020038
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Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027
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Malairuang, K., Krajang, M., Sukna, J., Rattanapradit, K., & Chamsart, S. (2020). High Cell Density Cultivation of Saccharomyces cerevisiae with Intensive Multiple Sequential Batches Together with a Novel Technique of Fed-Batch at Cell Level (FBC). Processes, 8(10), 1321. https://doi.org/10.3390/pr8101321
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Olivares-Marin, I. K., González-Hernández, J. C., Regalado-Gonzalez, C., & Madrigal-Perez, L. A. (2018). Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism. Journal of Visualized Experiments, 139. https://doi.org/10.3791/58192
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Bajić, B., Vučurović, D., Vasić, Đ., Jevtić-Mučibabić, R., & Dodić, S. (2022). Biotechnological Production of Sustainable Microbial Proteins from Agro-Industrial Residues and By-Products. Foods, 12(1), 107. https://doi.org/10.3390/foods12010107
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Rajput, S. D., Pandey, N., & Sahu, K. (2024). A comprehensive report on valorization of waste to single cell protein: strategies, challenges, and future prospects. Environmental Science and Pollution Research, 31(18), 26378–26414. https://doi.org/10.1007/s11356-024-33004-7
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Yang, S., Song, L., Wang, J., Zhao, J., Tang, H., & Bao, X. (2024). Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins. Engineering Microbiology, 4(1), 100122. https://doi.org/10.1016/j.engmic.2023.100122
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